Journal: Clinical and Translational Radiation Oncology

Article Title: Differential regulation of radioadaptation by quercetin between human normal and cancer cells

doi: 10.1016/j.ctro.2025.101099

Figure Lengend Snippet: Quercetin restores NRF2 nuclear translocation in radioadapted MCF10A cells. Representative immunofluorescence images of NRF2 (green) and DAPI (blue) and quantification of the nuclear-to-cytoplasmic NRF2 fluorescence ratio in MCF10A cells 24 h after 5 Gy irradiation with or without prior LDRT and quercetin treatment (Scale bar: 10 μm). Data is shown as mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: Cells were incubated with rabbit anti-human NRF2 primary antibody (1:200; Proteintech, 16396–1-AP), and detection was performed using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Translocation Assay, Immunofluorescence, Fluorescence, Irradiation

Journal: Journal of Advanced Research

Article Title: Adaptive bilirubin nanoscavenger alleviates pulmonary oxidative stress and inflammation for acute lung injury therapy

doi: 10.1016/j.jare.2025.03.027

Figure Lengend Snippet: The ROS-scavenging ability of GP@BR. A) DCF fluorescence images of A549 cells after various treatments, scale = 100 μm. B) Quantitative results of DCF fluorescence. C) The flow cytometry results show the intracellular ROS level. D) Histogram presents the flow cytometry results. E) Western blot results for Nrf2, Keap1, HO-1, and IL-1β in each group. Tubulin was used as an internal control; F)-I) Quantitative western blot results of F) Nrf2, G) Keap1, H) HO-1, and I) IL-1β relative to Tubulin. Data are presented as mean ± SD (n = 3); ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, indicating comparison with the H 2 O 2 group or between groups.

Article Snippet: Primary antibodies to Keap1 (WL03285) were obtained from Wanlei Biotechnology Co., Ltd. (Shenyang, China); Primary antibodies to Nrf2 (16396–1-AP) and HO-1 (66743–1-Ig) were obtained from Proteintech Group (Wuhan, China); Primary antibodies to IL-6 (DF6087), IL-1β (AF5103), β-actin (AF7018) and β-tubulin (AF7011) were obtained from Affinity Biosciences (Jiangsu, China).

Techniques: Fluorescence, Flow Cytometry, Western Blot, Control, Comparison

Journal: Journal of Advanced Research

Article Title: Adaptive bilirubin nanoscavenger alleviates pulmonary oxidative stress and inflammation for acute lung injury therapy

doi: 10.1016/j.jare.2025.03.027

Figure Lengend Snippet: The in vivo anti-inflammatory and antioxidant capabilities of GP@BR. The concentrations of (A) IL-6 and (B) IL-1β in BALF. (C) Immunoblot results for HO-1 in lung tissues, β-actin was used for internal control. (D) Immunohistochemical analysis of IL-6, IL-1β, Nrf2, and Keap1 in lung tissues. Scale bar = 100 μm. Data are presented as mean ± SD (n = 3), ** P < 0.01, **** P < 0.0001, indicating statistical significance compared to the ALI model group.

Article Snippet: Primary antibodies to Keap1 (WL03285) were obtained from Wanlei Biotechnology Co., Ltd. (Shenyang, China); Primary antibodies to Nrf2 (16396–1-AP) and HO-1 (66743–1-Ig) were obtained from Proteintech Group (Wuhan, China); Primary antibodies to IL-6 (DF6087), IL-1β (AF5103), β-actin (AF7018) and β-tubulin (AF7011) were obtained from Affinity Biosciences (Jiangsu, China).

Techniques: In Vivo, Western Blot, Control, Immunohistochemical staining

Journal: Frontiers in Pharmacology

Article Title: Coreopsis tinctoria Nutt. attenuates ultraviolet A photodamage by suppressing endoplasmic reticulum stress-induced apoptosis via Nrf2 crosstalk

doi: 10.3389/fphar.2025.1686234

Figure Lengend Snippet: CT alleviates UVA-induced ER stress and apoptosis via Nrf2 in HaCaT cells. (A) HaCaT cells were subjected to UVA radiation and treated with 50 or 100 μg/mL CT and/or siNrf2. Western blot assays were performed to detect the levels of ATF6, phosphorylated elF2a (p-elF2a), CHOP, GADD34, Cleaved caspase9, Cleaved caspase3, JNK, phosphorylated JNK (p-JNK), Bax, BCL2, cytoplasmic and nuclear NRF2 and HO-1. (B) Flow cytometry analysis was performed to determine cell apoptosis. Quantification of the percentage of apoptotic cells in different treatments. (C) Heatmap generated the data from RNAseq. Genes are all related to NRF2 downstream anti-oxidant or detoxification target genes. The figure was generated by SRplot . N = 4. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: After incubation with an endogenous peroxidase blocker (ZSGB-BIO, PV-6000), the slides were blocked with 3% BSA for 1 h. Primary antibodies against NRF2 (Proteintech, 16396-1-AP), Caspase12 (Proteintech, 55238-1-AP), and CHOP (Proteintech, 15204-1-AP) were incubated overnight at 4 °C at a dilution of 1:200 each.

Techniques: Western Blot, Flow Cytometry, Generated

Journal: Frontiers in Pharmacology

Article Title: Coreopsis tinctoria Nutt. attenuates ultraviolet A photodamage by suppressing endoplasmic reticulum stress-induced apoptosis via Nrf2 crosstalk

doi: 10.3389/fphar.2025.1686234

Figure Lengend Snippet: Therapeutic effects of CT on macroscopic and histological changes induced by ER stress in UVA-photodamaged mouse skin. (A) Schematic representation of the experimental design for mice study. (B) Representative pictures of untreated or treated with UVA with CT or not. (C) Representative pictures of H&E and Masson staining of the skin of mice from different treatment groups on day 14. (D–F) Representative images of CHOP, NRF2, and caspase-12 for IHC analysis performed on skin sections of mice from different treatment groups on day 14.

Article Snippet: After incubation with an endogenous peroxidase blocker (ZSGB-BIO, PV-6000), the slides were blocked with 3% BSA for 1 h. Primary antibodies against NRF2 (Proteintech, 16396-1-AP), Caspase12 (Proteintech, 55238-1-AP), and CHOP (Proteintech, 15204-1-AP) were incubated overnight at 4 °C at a dilution of 1:200 each.

Techniques: Staining